Hate to break it to you, but a Queen in 1v1 vs Regulus is rarely going to be in your favor.  Bring your teammate to attempt a burst or avoid her.

I played alongside Siothurn this past weekend, and he is just a beast with QoT. Normally I end up with the bulk of the awards, but he left me in the dust. I think it helped that our enemies got the matchups backwards. Instead of the enemy UB ripping apart my fragile TB, and the tower Rook possibly stonewalling the Queen, most of the time it was the UB staring at the word "Absorbed" and the Rook watching his towers get AoE'd to pieces.

CosMoe interesting suggestion, but unfortunately that's what I tried to do.  I had serpent for favor and was laying down mines like it was going out of style, and casting mark of betrayer whenever I could, but what would usually happen is mark goes off one mine goes off, he get's hit a little, maybe some minions die, but he barely gets scratched as shield absorbs a tonne of dmg.  Then he just recasts shield and uses hol and he's back to full everything and taking down your towers with uproot/violent siege.

I think the problem is that in 2v2 the shield stays up long enough to absorb all the dmg you can throw at it, as two heroes don't do enough dps to get it down to where QOT is in any kind of danger.  Also if you avoid her completely she pushes you lanes down so quickly the game will be over in 2 secs.  The main problem is that not only is she way to strong from lvl 1 (I would like to see generals require time to get stronger like oak), but with just one HOL and maybe some speed boots, there is no way to take her out efficiently (sure you could get two heroes on her and go all out and maybe you will kill her, but you won't make it out allive as the other hero mops both of you up very easily.  So you essentially are reduced to laying mines all over the place moping up creeps and out lvl'ing them but it means nothing really as once you finally get to a point where you have lots of gold and are pushing them back, you are war rank 6 they are 10, and here come the dark giants with lots of good upgrades.  Add to this the fact that towers don't do a good job on targeting the heroes over creeps, and you realise that she can sit in your base with 4 towers pounding on her while she captures your portal flag and caps it.  She then resummons moves to the other side, guards that portal and then it's you and your ally vs all the portals worth of dark giants.  It's only a matter of time after that (even with a million mines) before you citadel goes kaboom.

Maybe if mark was available earlier it could make a difference but 600 aoe dmg at lvl 10 is nothing at that point when you consider that lvl 1 shield absorbes more dmg, and if the creeps die she can do a full resummon and then hol and be back to full everything.  I'm sure in 4v4 and 5v5 she's weak as a 2 or 3 person gank probably results in a quick death and her minions are probably worthless when there are 2plus heroes per spawn lane.  2v2 though she is deadly, and near impossible to beat.  I'm hoping that someone that has any kind of experience with this type of situation can tell me there secret.  Then again Siothrun hasn't lost yet in pantheon and I can see why.

Now this I hadn't considered, it looks like this could work even better then parasitic egg, is a lot cheaper, and the mana drain is nice (then again HOL regens there mana faster) but if I beline to this item I should be able to get it before then can get HOL so this might just work.  I'll have to try this in my next game.

Warlord's Punisher seems too unreliable with a 30 second cooldown, and 400 mana every 30 seconds ain't all that big of a deal. It MAY work, if you coupled it with Gauntlets of Despair (15% chance to drain 100 mana) - will need high attack rate for it to really work. If he's able to just summon minions over and over, it means he's playing with a huge mana pool. From what you described of your game, his minions aren't the problem, it's the inability to deal enough damage to him, which makes me doubt the usefulness of Parasitic Egg (although if he summons more minions then it uses more mana). Maybe getting Staff of Renewal so you can lay down more mines and Marks.

Otherwise, I'm just as stumped.

(Deathbringer allows you to silence a target, but it's a 20k artifact, lol, probably not something to base a strategy around.)

Edit: I just realize this thread is about how to win vs QoT not help with win with QoT lol... man, that's what hunger do to you, but at least you know things from QoT's point of view. So now I will put below in quote.

A combination of mark of betrayer -> mines -> heaven's wrath can create a good burst damage. However, without some potion and high armor, you really will be risking alot in that spot against almost any general.

One thing QoT doesn't have is disabling skills (stun/interrupt), so if you are in trouble, you can always tele back without problem. That's one thing that annoy QoT player all the time, she can't do anything against foe in flee.

Locking flag and running away is an effective way to waste QoT's time.

Go full slow stacking bug absue and HP (carry some pots) and you will win. Even with no skill.

Be carefull trying to run a war of attrition with QoT. Her shield and mulch are much more mana efficiant than attack abilitys dammage ( highest mulch costs around 450 to summon and mulch neting 2250 health). However her inability to disable her foes is a big weakness. Stay away from her untill you can double team her (preferably when you just beat her team mate so he cant come help), spread out to avoid spike wave slowing both of you at the same time and open up with as much burst as you can.  Every time you fail to kill her you will lose more strength than she does in the engagement becouse of her efficiancy, so only engage when you can win ie. 2 v 1.  Your advantage is you can almoast always get away from her and she can only try to outlive the assault. Tower Rook also goes realy well at holding her advance, but your playing reg so it's not that relevant. If you can survive to the late game (over lv 15 she starts rapidly loosing effectiveness compared to most demigods) and havent fell to far behind you can definately turn it around.  Be aware though many QoT know this and after buying their initial gear will be saving for giants as they know they arent as good at personal combat in the late game. Be prepared to (Gasp) sell some of your items in order to match her giants if you must. I hope some of this may be helpfull.

this is the main problem in games with less then 3-5 people per side. In a 2v2 situation you have to avoid her as you cannot win the war of attrition with her.  This means she can essentially keep the flags under her control (on her side at the very least).  So you hang back near the tower trying to lvl up as quickly as possible.  the problem is while you will eventually pass the QOT in terms of levels mid-late game, she has her item set up for next to nothing, had map control for most of the game (so higher warscore), and can buy piles of citadel upgrades all at once.  Once the citadel upgrades are in place (and you can't match because your warscore isn't high enough) they will start to push the base.  This is where all the creeps they bought, along with the summongs, and her ability to destroy towers and shield herself let her march right in and cap your bases portals.  Nothing you can do about it with reg, as you can't get close to her without dying, and staying back and firing at range and throwing mines, will not prevent her from caping your portal.  It's just a very difficult matchup and the problem seems to be right from lvl 1 there is no way to kill her out of the box.

In 3v3+ games you could always get enough burst dmg to negate her shield and pwn her 3v1, but in 2v2 even if you get her 2v1 I don't see you doing enough dmg to move her from the lane, or whatever else she is trying to do.  The exception i could see would be a tower rook but since you don't know your allies before hand it would be nice to have something to deal with her better.  Mines seem to be the best solution in terms of skills, now just trying to find the best items to take her down.